Premium
cDNA arrays: Gene expression profiles of Hodgkin's disease and anaplastic large cell lymphoma cell lines
Author(s) -
Thorns Christoph,
Gaiser Timo,
Lange Karin,
Merz Hartmut,
Feller Alfred C.
Publication year - 2002
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1046/j.1320-5463.2002.01400.x
Subject(s) - complementary dna , anaplastic large cell lymphoma , gene , gene expression , gene expression profiling , biology , lymphoma , anaplastic lymphoma kinase , cell culture , large cell lymphoma , large cell , cancer research , microbiology and biotechnology , cell , genetics , pathology , immunology , cancer , medicine , adenocarcinoma , malignant pleural effusion , lung cancer
cDNA arrays are a powerful tool for the identification of differentially expressed genes in malignant tumors. We used this technique to study the gene expression profiles of anaplastic large cell lymphoma (ALCL) and Hodgkin's disease (HD). Gene expression of 11 lymphoma cell lines was analyzed covering 1176 cDNA sequences. Comparing these data to the expression profiles of B‐ and T‐lymphocytes, we identified 27 genes that were deregulated in all cell lines or in a particular entity. For the establishment of gene expression profiles the 27 genes were assigned to four groups composed of genes deregulated in (i) all lymphoma cell lines, (ii) ALCL and HD, (iii) only HD, and (iv) ALCL exclusively. Our results indicate that ALCL and HD share the differential expression of at least five genes. In addition, both entities are characterized by the differentially deregulated expression of four genes in HD and seven genes in ALCL. Because the expression profiling was performed on cell lines, further studies are needed to clarify the biological significance of the differentially expressed genes.