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Characterization of an Aedes aegypti bacterial artificial chromosome (BAC) library and chromosomal assignment of BAC clones for physical mapping quantitative trait loci that influence Plasmodium susceptibility
Author(s) -
Jiménez L. V.,
Kang B.K.,
DeBruyn B.,
Lovin D. D.,
Severson D. W.
Publication year - 2004
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.0962-1075.2004.00456.x
Subject(s) - biology , bacterial artificial chromosome , genetics , plasmodium gallinaceum , genome , quantitative trait locus , aedes aegypti , genomic library , insert (composites) , chromosome , cloning (programming) , primer (cosmetics) , gene , plasmodium falciparum , malaria , botany , mechanical engineering , chemistry , organic chemistry , immunology , computer science , gametocyte , base sequence , programming language , engineering , larva
Previous studies have confirmed a genetic basis for susceptibility of mosquitoes to Plasmodium parasites. Here we describe our efforts to characterize a bacterial artificial chromosome genomic library for the yellow fever mosquito, Aedes aegypti , and to identify BAC clones containing genetic markers that define quantitative trait loci (QTL) for Plasmodium gallinaceum susceptibility. This library (NDL) was prepared from the Ae. aegypti Liverpool strain and consists of 50 304 clones arrayed in 384‐well microplates. We used PCR analysis with oligonucleotide primer pairs specific to 106 genetic markers (as sequence‐tagged sites or STS) to screen the NDL library. Each STS identified between one and thirteen independent clones with an average of 3.3 clones. The average insert size was 122 kb and therefore the NDL library provides approximately 7.87‐fold genome coverage. The availability of the NDL library should greatly facilitate physical mapping efforts, including positional cloning of QTL for traits of interest such as Plasmodium susceptibility and for whole genome sequence determination and assembly.