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Identification and characterization of a Drosophila homologue of ATBP
Author(s) -
Aozasa N.,
Kubo Takeo,
Sekimizu Kazuhisa,
Natori Shunji
Publication year - 2002
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.0962-1075.2001.00304.x
Subject(s) - biology , drosophila (subgenus) , gene , reporter gene , microbiology and biotechnology , peptide sequence , genetics , drosophilidae , conserved sequence , drosophila melanogaster , gene expression
ATBP [(A+T)‐stretch binding protein] activates the Sarcophaga defense protein genes in an (A+T)‐stretch dependent manner as shown previously using a luciferase reporter assay [Aozasa et al . (2001) Eur J Biochem, 268, 2506–2511]. We identified the Drosophila homologue of ATBP. Drosophila ATBP has 388 amino acid residues and the amino acid sequence has high similarity with that of the Sarcophaga ATBP. In particular, residues 1–56 and 301–374 are highly conserved between Sarcophaga and Drosophila . Drosophila ATBP activated the Sarcophaga lectin gene promoter in SL‐2 cells. The Drosophila ATBP gene is a single copy gene and is located in the 20E region of chromosome X.