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Functional expression of a locust tyramine receptor in murine erythroleukaemia cells
Author(s) -
Poels J.,
Suner M.M.,
Needham M.,
Torfs H.,
De Rijck J.,
De Loof A.,
Dunbar S. J.,
Vanden Broeck J.
Publication year - 2001
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.0962-1075.2001.00292.x
Subject(s) - tyramine , biology , agonist , receptor , signal transduction , locust , forskolin , g protein coupled receptor , octopamine (neurotransmitter) , biochemistry , microbiology and biotechnology , endocrinology , medicine , botany , serotonin
The LCR/MEL system (Locus Control Region/Murine Erythroleukaemia cells) was employed to express and characterize the Locusta migratoria tyramine receptor (Tyr Loc ), an insect G protein‐coupled receptor. Functional agonist‐dependent responses were recorded in stable, tyramine receptor expressing cell clones (MEL‐Tyr Loc ). Tyramine elicited a dose‐dependent increase of cytosolic Ca 2+ ‐ions and an attenuation of forskolin‐induced cyclic adenosine monophosphate (AMP) production. Octopamine was shown to be a weak agonist for both responses. In addition, yohimbine proved to be a potent tyramine receptor antagonist. This study reports the first application of the LCR/MEL expression system in functional assays for G protein‐coupled receptors and therefore expands the capabilities of this system by exploiting the functionality of the signal transduction pathways.