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Effects of malaria infection on vitellogenesis in A nopheles gambiae during two gonotrophic cycles
Author(s) -
Ahmed A. M.,
Maingon R.,
Romans P.,
Hurd H.
Publication year - 2001
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.0962-1075.2001.00273.x
Subject(s) - biology , hemolymph , vitellogenesis , plasmodium yoelii , midgut , anopheles gambiae , blood meal , vitellogenin , parasite hosting , malaria , messenger rna , plasmodium (life cycle) , abundance (ecology) , medicine , andrology , endocrinology , immunology , zoology , microbiology and biotechnology , ecology , biochemistry , parasitemia , gene , plasmodium falciparum , oocyte , embryo , larva , world wide web , computer science
We report changes in the abundance of vitellogenin (Vg) mRNA, and concentration of haemolymph Vg and ovarian vitellin (Vn) in Anopheles gambiae following infection with Plasmodium yoelii nigeriensis . A parasite‐induced reduction in Vg mRNA abundance was first detected 24 h after feeding on an infective blood meal, when ookinetes were invading the midgut. During a second gonotrophic cycle post‐infection, developing oocysts reduced Vg mRNA abundance by up to 33% and the effect was detected from 2 h post blood meal. Concentrations of Vg were initially reduced by infection during the second cycle, as predicted from Vg mRNA measurements. However, after 24 h, excess Vg had accumulated in the haemolymph. This accumulation may be due to impaired uptake, since ovarian vitellin accumulation was significantly decreased by infection during both gonotrophic cycles.