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Primary cell culture and morphological characterization of canine dermal papilla cells and dermal fibroblasts
Author(s) -
BratkaRobia Christine B.,
Mitteregger Gerda,
Aichinger Amanda,
Egerbacher Monika,
Helmreich Magdalena,
Bamberg Elmar
Publication year - 2002
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1046/j.0959-4493.2001.00276.x
Subject(s) - dermal papillae , laminin , dermis , staining , fibroblast , immunohistochemistry , pathology , major duodenal papilla , dermal fibroblast , explant culture , biology , cell culture , microbiology and biotechnology , chemistry , anatomy , in vitro , medicine , hair follicle , extracellular matrix , biochemistry , genetics
Skin biopsies were taken from female dogs, the primary hair follicles isolated and the dermal papilla dissected. After incubation in supplemented Amniomax complete C100 medium in 24‐well culture plates, the dermal papilla cells (DPC) grew to confluence within 3 weeks. Thereafter, they were subcultivated every 7 days. Dermal fibroblast (DFB) cultures were established by explant culture of interfollicular dermis in serum‐free medium, where they reached confluence in 10 days. They were subcultivated every 5 days. For immunohistochemistry, cells were grown on cover slips for 24 h, fixed and stained with antibodies against collagen IV and laminin. DPC showed an aggregative growth pattern and formation of pseudopapillae. Intensive staining for collagen IV and laminin could be observed until the sixth passage. DFB grew as branching, parallel lines and showed only weak staining for collagen IV and laminin.