Premium
Liquid‐based cytology as a tool for the performance of uCyt+ TM and Urovysion ® Multicolour‐FISH in the detection of urothelial carcinoma
Author(s) -
Mian C.,
Lodde M.,
Comploj E.,
Negri G.,
EgarterVigl E.,
Lusuardi L.,
Palermo S.,
Marberger M.,
Pycha A.
Publication year - 2003
Publication title -
cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 48
eISSN - 1365-2303
pISSN - 0956-5507
DOI - 10.1046/j.0956-5507.2003.00094.x
Subject(s) - cytology , fish <actinopterygii> , urine cytology , medicine , liquid based cytology , urothelial cancer , urinary system , pathology , urothelial carcinoma , cancer , bladder cancer , biology , cervical cancer , fishery
The aim of the study was to assess the value of liquid‐based urinary cytology as a tool to perform uCyt+ and Multicolour‐FISH in patients under follow‐up after urothelial cancer. Therefore, standard cytology was compared to liquid‐based cytology with the addition of the uCyt+ test, which traces the three monoclonal antibodies M344, LDQ10 and 19A211 in exfoliated urothelial cells; and Multicolour‐FISH (including centromere‐specific probes for chromosomes 3, 7, 17 and a locus‐specific probe for 9p21/p16) performed on thin‐layer specimens. UCyt+ showed an overall sensitivity of 86.2% and cytology of 45.0%. Overall sensitivity of both the tests combined was 90%. Sensitivity of Multicolour‐FISH was 96.4%. All conventional cytology diagnoses were confirmed by liquid‐based cytology. Liquid‐based cytology is a valid tool for the performance of adjunctive analyses, such as uCyt+ and Multicolour‐FISH, on residual cellular material.