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Temporally controlled targeted somatic mutagenesis in the mouse brain
Author(s) -
Weber Philipp,
Metzger Daniel,
Chambon Pierre
Publication year - 2001
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1046/j.0953-816x.2001.01803.x
Subject(s) - cre recombinase , genetically modified mouse , transgene , biology , somatic cell , gene targeting , mutagenesis , neuroscience , microbiology and biotechnology , reporter gene , genetics , gene , mutation , gene expression
To develop spatio‐temporally controlled somatic mutagenesis in the adult mouse nervous system, we established transgenic mice expressing the tamoxifen‐inducible Cre‐ER T recombinase under the control of the mouse prion protein (PrP) promoter. Cre‐ER T was expressed in most regions of the brain and in the retina of one transgenic line, whereas its expression was mostly restricted to the hippocampus and the cerebellum in another line. As tamoxifen efficiently induced Cre‐mediated recombination in the various neuronal cell types expressing Cre‐ER T in the brain of adult mice, the PrP‐Cre‐ER T lines should be valuable tools to study the functions of genes involved in neurodegenerative diseases or regeneration, and in complex processes such as behaviour, learning and memory. Some limitations of presently available reporter lines for Cre‐mediated recombination in adult mouse CNS are discussed.