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Feeding suppression by fibroblast growth factor‐1 is accompanied by selective induction of heat shock protein 27 in hypothalamic astrocytes
Author(s) -
Suzuki Seigo,
Li AiJun,
Ishisaki Akira,
Hou Xiaogang,
Hasegawa Mamoru,
Fukumura Masayuki,
Akaike Toshihiro,
Imamura Toru
Publication year - 2001
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1046/j.0953-816x.2001.01606.x
Subject(s) - hsp27 , medicine , endocrinology , astrocyte , fibroblast growth factor , hypothalamus , biology , neurotrophic factors , basic fibroblast growth factor , leptin , ventricle , growth factor , heat shock protein , hsp70 , microbiology and biotechnology , central nervous system , gene , genetics , receptor , obesity
It has been suggested that fibroblast growth factor (FGF)‐1 serves as a physiological satiety factor in the hypothalamus, although the molecular mechanism underlying such a function is poorly understood. To gain additional insight into this issue, we used a Sendai virus (SeV) gene expression system in rats to explore genes differentially expressed subsequent to expression of FGF‐1. Using cDNA arrays, we determined that infusion of FGF‐1/SeV into one lateral ventricle induced selective expression of heat shock protein (HSP) 27 in the hypothalamus. Whereas FGF‐1 expression was restricted to the ependymal cell layer of the cerebral ventricles, HSP27 was more widely expressed in astrocytes residing in the surrounding periventricular region. Similarly, infusion of FGF‐1 polypeptide into a lateral ventricle induced dose‐dependent HSP27 expression in periventricular astrocytes surrounding the third ventricle, with maximum mRNA levels being attained 6 h after infusion. This induction of HSP27 was accompanied by a significant suppression of feeding behaviour. Interestingly, suppression of feeding caused by intracerebro ventricular infusion of ciliary neurotrophic factor was also accompanied by induction of HSP27 in periventricular astrocytes, but suppression of feeding caused by infusion of leptin was not. It therefore appears that suppression of feeding by FGF‐1 is accompanied by selective induction of HSP27 expression in hypothalamic astrocytes surrounding the third ventricle, and that this response may be a key component of the mechanism by which appetite is regulated by FGF‐1.