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Cajal‐Retzius cells in early postnatal mouse cortex selectively express functional metabotropic glutamate receptors
Author(s) -
MartínezGalán Juan Ramón,
LópezBendito Guillermina,
Luján Rafael,
Shigemoto Ryuichi,
Fairén Alfonso,
Valdeolmillos Miguel
Publication year - 2001
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1046/j.0953-816x.2001.01494.x
Subject(s) - metabotropic glutamate receptor , metabotropic glutamate receptor 1 , neuroscience , metabotropic glutamate receptor 5 , metabotropic glutamate receptor 6 , metabotropic glutamate receptor 7 , glutamate receptor , metabotropic glutamate receptor 2 , agonist , metabotropic receptor , biology , chemistry , receptor , biochemistry
Glutamate receptors have been linked to the regulation of several developmental events in the CNS. By using cortical slices of early postnatal mice, we show that in layer I cells, glutamate produces intracellular calcium ([Ca 2+ ] i ) elevations mediated by ionotropic and metabotropic glutamate receptors (mGluRs). The contribution of mGluRs to these responses was demonstrated by application of tACPD, an agonist to groups I and II mGluRs, which evoked [Ca 2+ ] i increases that could be reversibly blocked by MCPG, an antagonist to groups I and II mGluRs. In the absence of extracellular Ca 2+ , repetitive applications of tACPD or quisqualate, an agonist to group I mGluRs, elicited decreasing [Ca 2+ ] i responses that were restored by refilling a thapsigargin‐sensitive Ca 2+ store. The use of specific group I mGluR agonists CHPG and DHPG indicated that the functional mGluR in layer I was of the mGluR1 subtype. Subtype specific antibodies confirmed the presence of mGlur1α, but not mGluR5, in Cajal‐Retzius (Reelin‐immunoreactive) neurons.