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Genetische Variabilität am CSN1S2 Genort bei zwei vom Aussterben bedrohten sizilianischen Ziegenrassen
Author(s) -
Marletta D.,
Bordonaro S.,
Guastella A. M.,
D'Urso G.
Publication year - 2004
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1046/j.0931-2668.2003.00413.x
Subject(s) - biology , genotype , allele , null allele , locus (genetics) , polymerase chain reaction , genetics , veterinary medicine , gene , medicine
Summary In this study we surveyed two endangered Sicilian goat breeds (Girgentana and Argentata dell'Etna) for genetic polymorphism at the CSN1S2 locus. In a total of 537 goats, we detected CSN1S2 A , CSN1S2 B , CSN1S2 C° (CSN1S2 C + CSN1S2 E ), CSN1S2 D , CSN1S2 F and CSN1S2 O alleles by means of various polymerase chain reaction (PCR), allele specific‐PCR (AS–PCR) and PCR‐restriction fragment length polymorphism (RFLP) reactions with the aim of improving the knowledge of the genetic resource of these two breeds. Three and five alleles, with six and twelve genotypes, were identified at CSN1S2 locus, in Girgentana and Argentata, respectively. Argentata dell'Etna showed a higher degree of genetic variation. The allelic and genotypic distribution seems to be significantly different (p < 0.001) in the two breeds. In Argentata the rare null allele (CSN1S2 O ) was found at low frequency (0.033); this genetic peculiarity makes its preservation worthwhile.