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Ouabain‐sensitive respiration and protein synthesis in duodenal mucosa and liver in rats fed increasing levels of pea fibre or protein and housed in 18 or 28°C environments
Author(s) -
Zhao XinQuan,
Jørgensen H.,
Gabert V. M.
Publication year - 2000
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1046/j.0931-2439.2000.00295.x
Subject(s) - respiration , ouabain , chemistry , medicine , endocrinology , zoology , biology , sodium , anatomy , organic chemistry
Summary Seventy‐two Wistar rats were used in two studies to investigate the effect of environmental temperature (18 or 28°C), and increasing levels of dietary fibre (low, 68 g/kg dry matter (DM); medium 110 g/kg DM; high, 157 g/kg DM) and protein (low, 91 g/kg DM; medium, 171 g/kg DM; high, 262 g/kg DM) on respiration attributable to Na + ,K + ‐ATPase activity and protein synthesis in duodenal mucosa and liver of rats. In vitro O 2 consumption in tissues was measured polarographically using a Clark‐style YSI biological O 2 monitor. Whole‐body O 2 consumption was measured with two open‐circuit respiration chambers. Whole‐body O 2 consumption was higher (p < 0.05) at 18°C than at 28°C. Rats fed the low protein diet had significantly higher (p < 0.05) whole‐body O 2 consumption than those fed the medium or high protein diet. Compared with 28°C, the environmental temperature of 18°C caused an increase (p < 0.05) in total O 2 consumption and O 2 consumption attributable to Na + ,K + ‐ATPase activity in duodenal mucosa. There was no effect (p > 0.05) of environmental temperature on total O 2 consumption, Na + ,K + ‐ATPase activity attributable to protein synthesis dependent on O 2 consumption in the liver. Total O 2 consumption and O 2 consumption attributable to Na + ,K + ‐ATPase activity increased (p < 0.05) in duodenal mucosa in rats fed the low level of dietary fibre compared with rats fed the medium level of dietary fibre. In vitro O 2 consumption determined in duodenal mucosa and in liver did not always correspond to whole‐body O 2 consumption. This may indicate that respiration in the duodenum and liver adapts differently and may not reflect changes in whole‐body respiration in response to dietary modification and changes in thermal environment.