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Adhesion of a chondrocytic cell line (USAC) to fibronectin and its regulation by proteoglycan
Author(s) -
Imoto Emi,
Kakuta Saburo,
Hori Mayumi,
Yagami Kimitosi,
Nagumo Masao
Publication year - 2002
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1046/j.0904-2512.2001.00202.x
Subject(s) - fibronectin , proteoglycan , extracellular matrix , integrin , microbiology and biotechnology , cell adhesion , cartilage , cell culture , laminin , aggrecan , cell adhesion molecule , chemistry , cell , biology , anatomy , biochemistry , pathology , medicine , genetics , articular cartilage , alternative medicine , osteoarthritis
Background: Chondrocytes produce various extracellular matrices during chondrogenesis. Fibronectin and proteoglycan are major extracellular matrix proteins in cartilage tissue, but the interactions between them are not clear. Methods: Recently, we succeeded in establishing a cell line (USAC) with phenotypes of chondrocytes from a human osteogenic sarcoma of the mandible. Using this cell line, cell adhesion to fibronectin, the effect of proteoglycan on the cell adhesion and expression of integrin α5β1 were investigated. Results: Cells immediately adhered to fibronectin and then spread. Proteoglycan inhibited cell adhesion to fibronectin dose‐dependently, whereas collagen did not. The expression of both mRNAs of α5 and β1 subunits was detected 12 h after treatment with proteoglycan, but the expression of β1 subunit mRNA had diminished by 24 h after treatment. Conclusions: These findings suggest that proteoglycan might modulate signal transduction from fibronectin by decreasing the expression of α5β1 integrin.