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Procollagen 1 expression in atypical fibroxanthoma and other tumors
Author(s) -
Jensen Kristin,
Wilkinson Barbara,
Wines Nina,
Kossard Steven
Publication year - 2004
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1046/j.0303-6987.2004.0145.x
Subject(s) - atypical fibroxanthoma , pathology , immunohistochemistry , staining , histology , basal cell carcinoma , trichoepithelioma , medicine , basal cell
Background: Procollagen (PC) is secreted by fibroblasts into the extracellular matrix, where it is cleaved to form collagen. The rat anti‐human PC‐1 monoclonal antibody has been reported to react with atypical fibroxanthoma (AFX), a poorly differentiated but usually benign skin lesion common in elderly patients. We have studied PC‐1 staining in 50 tumors with AFX histological features (four of which were subsequently reclassified as non‐AFX tumors) to confirm this prior observation. In addition, we have investigated PC‐1 in other skin tumors, particularly those with spindled cell or sclerosing/desmoplastic morphologies. Method: Archival material was retrieved and sections were prepared and immunostained with PC‐1 as well as a panel of antibodies, including S‐100 and MNF‐116 (cytokeratins 5, 6, 7, 8, 17, and 19). Results: PC‐1 staining was strongly positive in 40 of 46 (87%) AFXs. Three AFXs displayed weak staining with PC‐1 even after repeat staining of 10 tumors that were initially weak. Three additional tumors stained with both PC‐1 and MNF‐116 and were classified as AFX‐like squamous cell carcinoma (SCC). One tumor with AFX‐like histology was PC‐1 negative and S‐100 positive and was classified as an AFX‐like melanoma. Positive staining in tumor cells was observed in three of nine (33%) desmoplastic malignant melanomas, three of eight (38%) desmoplastic SCCs, zero of 10 (0%) desmoplastic trichoepitheliomas, zero of 10 (0%) morpheic basal cell carcinomas, and zero of 10 (0%) sclerosing sweat duct carcinomas. Conclusion: PC‐1 is a useful antibody in a diagnostic immunohistochemical panel when investigating AFX and AFX‐like tumors; however, good technical quality and careful interpretation are necessary when using a panel of antibodies, particularly to keratin and S‐100 protein, for optimal accuracy.