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Androgen metabolic response to indomethacin and the alkaline phosphatase inhibitor levamisole in fibroblasts
Author(s) -
Soory M.,
Tilakaratne A.
Publication year - 2003
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1046/j.0303-6979.2003.00428.x
Subject(s) - alkaline phosphatase , chemistry , androgen , endocrinology , medicine , levamisole , context (archaeology) , pharmacology , enzyme , biochemistry , biology , hormone , paleontology
Objectives: The aim of this investigation is to study the effects of indomethacin (I) and the alkaline phosphatase (ALP) inhibitor levamisole (L) on androgen 5 α ‐reductase expression in gingival and periosteal fibroblasts, in the context of repair in the periodontium. Chronically inflamed human gingival fibroblasts (HGF) were used to demonstrate the comparative effects of L on HGF and human oral periosteal fibroblasts (HPF). Material and Methods: Monolayer cultures of six cell lines of HPF of the fifth to ninth passage were incubated in duplicate with 14C‐testosterone/14C‐4‐androstenedione as substrates in Eagle's MEM; I was added at concentrations of 1 and 3 μ g/ml in the presence or absence of the established inhibitory concentration of 30 μ g/ml L and incubated for 24 h. The medium was solvent extracted for radioactive metabolites, separated by thin layer chromatography and quantified. Results: L caused 50% inhibition of 5 α ‐reductase and 17 β ‐hydroxysteroid dehydrogenase activity in HGF. In HPF, 5 α ‐reductase expression was enhanced by I with both substrates, by 65–76% ( n =6; p <0.01), inhibited by 30–50% ( n =6; p <0.01) with L and restored to control values in combination. Conclusion: Yields of androgen metabolites may be linked to ALP activity, with implications on healing, during adjunctive treatment of inflammatory periodontal disease with I.