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Chemokine and cytokine expression in murine intestinal epithelium following Nippostrongylus brasiliensis infection
Author(s) -
Rosbottom Anne,
Knight Pamela A.,
McLachlan Gerry,
Thornton Elizabeth M.,
Wright Steven W.,
Miller Hugh R. P.,
Scudamore Cheryl L.
Publication year - 2002
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1046/j.0141-9838.2001.00437.x
Subject(s) - nippostrongylus brasiliensis , chemokine , biology , mast cell , intestinal epithelium , epithelium , immunology , cytokine , monocyte , ccl20 , inflammation , immune system , chemokine receptor , genetics
Summary Infection of mice with the nematode parasite Nippostrongylus brasiliensis results in a well characterized intestinal mastocytosis with intraepithelial migration of mucosal mast cells (MMC). The molecules mediating this response are unknown. We examined expression of several putative mast cell chemoattractants in intestinal epithelium following N. brasiliensis infection. Expression of the chemokines monocyte chemoattractant protein‐1 (MCP‐1), macrophage inflammatory protein‐1 α(MIP‐1α), RANTES (regulated on activation normal T‐cell expressed and secreted), fractalkine, and thymocyte expressed chemokine (TECK); and the cytokines stem cell factor (SCF) and transforming growth factor β 1 (TGFβ 1 ), was constitutive and no alteration was detected following infection. MCP‐1 expression was also constitutive but at much lower levels and increased expression was detected on days 7 and 14 postinfection. Expression of MCP‐1 in whole jejunum was at much higher levels than in epithelium. Constitutive expression of MCP‐1, MIP‐1α and TGFβ 1 was also detected in cultured bone marrow‐derived homologues of MMC. In an intestinal epithelial cell line (CMT‐93), there was constitutive expression of SCF, TGFα 1 , fractalkine and MCP‐1. The results show that, in vivo , epithelium is a potentially important source of mast cell chemoattractants.