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Evaluation of the HemoCue plasma haemoglobin analyser for assessing haemolysis in red cell concentrates during storage
Author(s) -
Cardigan R.,
Smith K.
Publication year - 2002
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.0042-9007.2001.00149.x
Subject(s) - haemolysis , analyser , coefficient of variation , red cell , chromatography , significant difference , chemistry , medicine , immunology
Background and Objectives To evaluate the HemoCue plasma haemoglobin (Hb) analyser for determining supernatant Hb in red cell concentrates (RCC) during storage and compare this to a spectrophotometric method. Materials and Methods Samples from RCC ( n = 20) at days 0, 7, 21, 35 and 42 of storage were tested by both methods for supernatant Hb. Results Results are given as median with range. There was a significant correlation ( r = 0·97, P < 0·0001), but statistically significant difference between the two methods (0·72 (0·36–2·77) spectrophotometric vs. 0·70 (0·3–2·6) g/l HemoCue). However, the mean bias was negligible at 0·06 g/l. The HemoCue method gave a interassay coefficient of variation of 5·6% at 1·2 g/l and was linear to at least 20 g/l. Conclusion The HemoCue plasma haemoglobin analyser is a simple, rapid, reliable and accurate method for the determination of supernatant Hb in RCC at the end of storage and compares well to established methods for supernatant Hb measurement.