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Promotion of neuronal cell adhesion by members of the IgLON family occurs in the absence of either support or modification of neurite outgrowth
Author(s) -
McNamee Christine J.,
Reed James E.,
Howard Mark R.,
Lodge Anthony P.,
Moss Diana J.
Publication year - 2002
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.0022-3042.2002.00798.x
Subject(s) - neurite , microbiology and biotechnology , transfection , cell adhesion molecule , dorsal root ganglion , cell adhesion , laminin , adhesion , neural cell adhesion molecule , cell culture , recombinant dna , biology , cell , chemistry , neuroscience , in vitro , extracellular matrix , spinal cord , biochemistry , genetics , organic chemistry , gene
The IgLONs are a family of glycosyl phosphatidyl inositol‐linked cell adhesion molecules which are thought to modify neurite outgrowth and may play a role in cell–cell recognition. The family consists of LAMP, OBCAM, neurotrimin/CEPU‐1 and neurotractin/kilon. In this paper we report the effect of recombinant LAMP, CEPU‐1 and OBCAM, and transfected cell lines expressing these molecules, on the adhesion and outgrowth of dorsal root ganglion (DRG) and sympathetic neurones. CHO cells transfected with cDNA for CEPU‐1 adhered to a recombinant CEPU‐1‐Fc substrate. However, DRG or sympathetic neurones only adhered to CEPU‐1‐Fc when presented on protein A. Although DRG and sympathetic neurones express IgLONs on their surface, both types of neurones exhibited differential adhesion to CEPU‐1‐Fc, LAMP‐Fc and OBCAM‐Fc. Neither DRG nor sympathetic neurones extended neurites on a protein A/IgLON‐Fc substrate and overexpression of CEPU‐1‐GFP in DRG neurones also failed to stimulate neurite outgrowth on an IgLON‐Fc substrate. DRG neurones adhered to and extended neurites equally on transfected and non‐transfected cell lines and the recombinant proteins did not modulate the outgrowth of neurones on laminin. In contrast to previous reports we suggest that IgLONs may not have a primary role in axon guidance but may be more important for cell–cell adhesion and recognition.