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Evidence for a γ‐hydroxybutyrate (GHB) uptake by rat brain synaptic vesicles
Author(s) -
Muller Claude,
Viry Sandrine,
Miehe Monique,
Andriamampandry Christian,
Aunis Dominique,
Maitre Michel
Publication year - 2002
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.0022-3042.2002.00780.x
Subject(s) - synaptic vesicle , gabaergic , neurotransmission , endogeny , population , inhibitory postsynaptic potential , biology , neurotransmitter , transporter , neuroscience , receptor , vesicle , chemistry , biochemistry , gene , medicine , environmental health , membrane
γ‐Hydroxybutyrate (GHB) is an endogenous metabolite of mammalian brain which is derived from GABA. Much evidence favours its role as an endogenous neuromodulator, synthesized, stored and released at particular synapses expressing specific receptors. One key step for GHB involvement in neurotransmission is its uptake by a specific population of synaptic vesicles. We demonstrate that this specific uptake exists in a crude synaptic vesicle pool obtained from rat brain. The kinetic parameters and the pharmacology of this transport are in favour of an active vesicular uptake system for GHB via the vesicular inhibitory amino acid transporter. This result supports the idea that GABA and GHB accumulate together and are coliberated in some GABAergic synapses of the rat brain, where GHB acts as a modulatory factor for the activity of these synapses following stimulation of specific receptors .