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Glucose deprivation and hypoxia increase the expression of the GLUT1 glucose transporter via a specific mRNA cis ‐acting regulatory element
Author(s) -
Boado Ruben J.,
Pardridge William M.
Publication year - 2002
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.0022-3042.2001.00756.x
Subject(s) - glut1 , glucose transporter , glucose transporter type 1 , endocrinology , hypoxia (environmental) , gene expression , medicine , reporter gene , biology , glucose uptake , messenger rna , regulation of gene expression , microbiology and biotechnology , chemistry , gene , biochemistry , insulin , organic chemistry , oxygen
The expression of the glucose transporter type‐1 (GLUT1) gene is up‐regulated in hypoxia and glucose deprivation. A 10 nucleotide (nt) cis ‐acting regulatory element (CAE), which is located within nt 2181–2190 of the GLUT1 3′‐untranslated region (CAE 2181−2190 ), increases the expression of a GLUT1‐luciferase reporter gene and decreases its mRNA decay. The present study investigated the role of the GLUT1 CAE 2181−2190 in glucose deprivation and hypoxia using stable transfectants. Glucose and O 2 deprivation produced a marked increase in the expression of the GLUT1 reporter gene carrying the CAE 2181−2190 , and this effect was additive. Glucose deprivation and/or hypoxia induced no significant changes in the expression of the reporter gene wherein the GLUT1 CAE 2181−2190 was site‐directed deleted. Data presented here suggest that the GLUT1 CAE 2181−2190 participates in the increase of GLUT1 gene expression in glucose deprivation and hypoxia.