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Up‐regulation of tissue‐type transglutaminase after traumatic
brain injury
Author(s) -
Tolentino Paul J.,
DeFord S. Michelle,
Notterpek Lucia,
Glenn Christopher C.,
Pike Brian R.,
Wang Kevin K. W.,
Hayes Ronald L.
Publication year - 2002
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.0022-3042.2001.00726.x
Subject(s) - tissue transglutaminase , western blot , northern blot , messenger rna , hippocampus , blot , cortex (anatomy) , microbiology and biotechnology , traumatic brain injury , biology , medicine , pathology , endocrinology , gene , biochemistry , neuroscience , enzyme , psychiatry
Tissue‐type transglutaminase (tTG, EC 2.3.2.13) has been implicated in various disease paradigms including neurodegenerative disease. In these studies, tTG induction after traumatic brain injury was studied using a rat cortical impact model. Using western blots, two forms of tTG protein expression were identified – a ∼79‐kDa primary form (tTG‐L) and a less abundant ∼70‐kDa form (tTG‐S). Both forms of tTG protein were elevated after injury. In ipsilateral cortex, peak induction of tTG‐L protein [561% ± 80% of control ( n = 5)] was observed five days after injury, with expression remaining elevated after two weeks. Peak induction of tTG‐S protein [302% ± 81% of control ( n = 5)] was observed three days after injury. Lesser tTG protein induction was observed in hippocampus. Northern blot analysis demonstrated two tTG transcripts in the ipsilateral cortex with peak induction of tTG‐L mRNA three days after injury. However, tTG‐S mRNA was not identified in control samples and only faintly detected in injured tissue. To facilitate analysis of low abundance transcripts in smaller tissue samples, a semiquantitative real‐time PCR strategy was used. Semi‐quantitative PCR analysis of tTG‐L mRNA induction in ipsilateral cortex (peak after three days; 414% ± 21% of control, n = 3) confirmed tTG‐L mRNA induction determined by northern blot (410% of control).