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Functional characterization of a novel serotonin receptor (5‐HT ap2 ) expressed in the CNS of Aplysia californica
Author(s) -
Barbas Demian,
Zappulla Jacques P.,
Angers Stéphane,
Bouvier Michel,
Castellucci Vincent F.,
DesGroseillers Luc
Publication year - 2002
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.0022-3042.2001.00703.x
Subject(s) - aplysia , 5 ht2a receptor , 5 ht receptor , biology , receptor , serotonin , ritanserin , ketanserin , 5 ht5a receptor , metergoline , 5 ht1 receptor , lymnaea , lymnaea stagnalis , receptor antagonist , microbiology and biotechnology , biochemistry , neuroscience , antagonist , ecology , snail
Serotonin has been shown to be a neuromodulator in the Aplysia californica CNS. The diversity of serotonin actions is due to the existence of several different receptor subtypes. In this study we report the cloning of a full‐length cDNA, coding for a novel serotonin receptor (5‐HT ap2 ). The receptor protein bears the characteristics of G protein‐coupled receptors. It shares 68% and 34% of its amino acid sequence identity with the 5‐HT lym receptor from Lymnaea stagnalis and the mammalian 5‐HT 1A receptor, respectively. When transfected in HEK 293 cells, 5‐HT ap2 was negatively coupled to adenylate cyclase. Ligand binding analysis indicated that the order of potencies of various drugs for the inhibition of [ 3 H]LSD binding was: methiothepin > metergoline > 5‐CT > PAPP > 5‐HT > ketanserin > NAN‐190 > 8‐OH‐DPAT > clozapine. RT‐PCR amplification of RNA isolated from different tissues indicated that this receptor is expressed in the CNS and in bag cells. The expression of 5‐HT ap2 restricted to the CNS suggests an important role for this receptor in the modulation of neuronal functions in Aplysia . Moreover, the high expression of 5‐HT ap2 in the bag cells, associated with its pharmacological profile, suggests that this receptor may be implicated in modulating the afterdischarge during the egg‐laying behavior.