Open AccessStructural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 1003
Author(s) -
Månsson Martin,
Hood Derek W.,
Li Jianjun,
Richards James C.,
Moxon E. Richard,
Schweda Elke K. H.
Publication year - 2002
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.0014-2956.2001.02707.x
Subject(s) - haemophilus influenzae , microbiology and biotechnology , strain (injury) , lipopolysaccharide , virology , biology , immunology , antibiotics , anatomy
Structural analysis of the lipopolysaccharide (LPS) of nontypeable Haemophilus influenzae strain 1003 has been achieved by the application of high‐field NMR techniques, ESI‐MS, capillary electrophoresis coupled to ESI‐MS, composition and linkage analyses on O‐deacylated LPS␣and core oligosaccharide material. It was found that␣the LPS contains the common structural element of␣ H. influenzae , l ‐α‐ d ‐Hep p ‐(1→2)‐[ P Etn→6]‐ l ‐α‐ d ‐Hep p ‐(1→3)‐[β‐ d ‐Glc p ‐(1→4)]‐ l ‐α‐ d ‐Hep p ‐(1→5)‐[ PP Etn→4]‐α‐Kdo p ‐(2→6)‐Lipid A, in which the β‐ d ‐Glc p residue is substituted by phosphocholine at O‐6 and an acetyl group at O‐4. A second acetyl group is located at O‐3 of the distal heptose residue (HepIII). HepIII is chain elongated at O‐2 by either a β‐ d ‐Glc p residue (major), lactose or sialyllactose (minor, i.e. α‐Neu5Ac‐(2→3)‐β‐ d ‐Gal p ‐(1→4)‐β‐ d ‐Glc p ), where a third minor acetylation site was identified at the glucose residue. Disialylated species were also detected. In addition, a minor substitution of ester‐linked glycine at HepIII and Kdo was observed.