Open AccessIsolation and characterization of photosystem II subcomplexes from cyanobacteria lacking photosystem I
Author(s) -
Szabò Ildikò,
Rigoni Fernanda,
Bianchetti Maria,
Carbonera Donatella,
Pierantoni Francesca,
Seraglia Roberta,
Segalla Anna,
Giacometti Giorgio M.
Publication year - 2001
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.0014-2956.2001.02441.x
Subject(s) - photosystem ii , thylakoid , photosynthetic reaction centre , photosystem i , cyanobacteria , photosynthesis , chemistry , light harvesting complexes of green plants , synechocystis , p700 , photochemistry , biology , biophysics , chloroplast , biochemistry , genetics , bacteria , gene
A photosystem II (PSII) core complex lacking the internal antenna CP43 protein was isolated from the photosystem II of Synechocystis PCC6803, which lacks photosystem I (PSI). CP47–RC and reaction centre (RCII) complexes were also obtained in a single procedure by direct solubilization of whole thylakoid membranes. The CP47–RC subcore complex was characterized by SDS/PAGE, immunoblotting, MALDI MS, visible and fluorescence spectroscopy, and absorption detected magnetic resonance. The purity and functionality of RCII was also assayed. These preparations may be useful for mutational analysis of PSII RC and CP47–RC in studying primary reactions of oxygenic photosynthesis.