Open AccessStructural studies of a cell wall polysaccharide of Trichosporon asahii containing antigen II
Author(s) -
Ichikawa Tomoe,
Nishikawa Akemi,
Ikeda Reiko,
Shinoda Takako
Publication year - 2001
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.0014-2956.2001.02438.x
Subject(s) - residue (chemistry) , chemistry , polysaccharide , mannose , trichosporon , cell wall , stereochemistry , mannan , acid hydrolysis , biochemistry , antigen , carbohydrate conformation , hydrolysis , yeast , biology , genetics
The structure of a cell‐wall polysaccharide containing antigen II from Trichosporon asahii was investigated. A purified glucuronoxylomannan (GXM) antigen was found to contain O ‐ acetyl groups that contribute to the serological reactivity. The structure of GXM was analyzed by partial acid hydrolysis, methylation analysis, controlled Smith degradation, NMR studies, and fluorophore‐assisted carbohydrate electrophoresis. GXM has an α‐(1→3)‐ d ‐mannan backbone with a β‐ d ‐glucopyranosyluronic acid residue bound to O‐2 of a mannopyranosyl residue and the same number of β‐ d ‐xylopyranosyl residues as mannose. Side chains of β‐ d ‐xylopyranosyl‐ d ‐xylopyranose, forming a nonreducing terminus, and β‐ d ‐xylopyranosyl residues were attached to O‐2, O‐4, and O‐6 of the mannose residues.