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Expression of the signal transduction molecule ζ in peripheral and tumour‐associated lymphocytes in Hodgkin's disease in relation to the Epstein–Barr virus status of the tumour cells
Author(s) -
Sjöberg Jan,
Andersson Margareta,
Garcia Carlos,
Palucka Karolina A.,
Björkholm Magnus,
PorwitMacDonald Anja,
Pisa Pavel
Publication year - 2002
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.0007-1048.2002.03342.x
Subject(s) - cd3 , cd16 , lymphocyte , immune system , immunohistochemistry , immunology , biology , cancer research , cd8
Summary. We investigated whether the described immune evasion of Epstein–Barr virus (EBV)‐infected malignant Hodgkin and Reed‐Sternberg (HRS) cells in Hodgkin's disease (HD) is paralleled by a disturbed expression of the signal transduction molecule ζ associated with CD3 and CD16 in tumour‐associated T lymphocytes (TAL). Flow cytometric analysis revealed a significantly lower ζ expression in CD3 + /4 + , CD3 + /8 + and CD16 + patient peripheral blood lymphocytes (PBL; n = 10) compared with normal donor PBLs ( n = 11). When patient PBLs were compared with the corresponding TAL, the latter showed a significantly higher (CD3 + /4 + ) or equal (CD3 + /8 + ) ζ expression. The EBV status of the tumours did not correlate with ζ expression in the TAL. Immunohistochemical staining revealed ζ‐positive lymphocytes among the adjacent bystander cells of the HRS cells in all analysed tumours ( n = 8), irrespective of tumour EBV status. In conclusion, these results do not support downregulation of ζ in TAL as a critical mechanism contributing specifically to the immune escape of EBV + HRS cells.