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Persistent polyclonal B lymphocytosis: an expansion of cells showing IgVH gene mutations and phenotypic features of normal lymphocytes from the CD27 + marginal zone B‐cell compartment
Author(s) -
Salcedo Irene,
CamposCaro Antonio,
Sampalo Almudena,
Reales Elena,
Brieva José A.
Publication year - 2002
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.0007-1048.2001.03327.x
Subject(s) - marginal zone , lymphocytosis , polyclonal antibodies , cd5 , biology , microbiology and biotechnology , phenotype , b cell , splenic marginal zone lymphoma , compartment (ship) , gene rearrangement , cd38 , somatic hypermutation , gene , antibody , genetics , immunology , flow cytometry , oceanography , stem cell , cd34 , geology
Summary. Persistent polyclonal B‐cell lymphocytosis (PPBL) is an unusual and benign lymphoproliferation characterized by a polyclonal expansion of B lymphocytes, whose nature remains undetermined. The phenotypic analysis of three cases revealed that these cells were CD27 + IgM high CD21 high CD5 low and CD23 low , a phenotype associated with the normal marginal zone (MZ) B‐cell compartment. As MZ B cells have initiated immunoglobulin (Ig)V gene somatic mutations, PPBL IgVH genes were sequenced. An average of 73% of these sequences were mutated. The mean number of mutation per sequence was 6·9, a number similar to those observed in the MZ B‐cell compartment.