Open AccessSteroid receptor-coregulator transcriptional complexes: new insights from CryoEM
Author(s) -
Ping Yi,
Xuelian Yu,
Zhao Wang,
Bert W. O’Malley
Publication year - 2021
Publication title -
essays in biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.351
H-Index - 66
eISSN - 1744-1358
pISSN - 0071-1365
DOI - 10.1042/ebc20210019
Subject(s) - androgen receptor , estrogen receptor , nuclear receptor , microbiology and biotechnology , computational biology , chromatin , receptor , biology , pelp 1 , histone , steroid , transcription (linguistics) , transcription factor , genetics , gene , biochemistry , hormone , linguistics , philosophy , prostate cancer , cancer , breast cancer
Steroid receptors activate gene transcription through recruitment of a number of coregulators to facilitate histone modification, chromatin remodeling, and general transcription machinery stabilization. Understanding the structures of full-length steroid receptor and coregulatory complexes has been difficult due to their large molecular sizes and dynamic structural conformations. Recent developments in cryo-electron microscopy (cryoEM) technology and proteomics have advanced the structural studies of steroid receptor complexes. Here, we will review the insights we learned from cryoEM studies of the estrogen and androgen receptor transcriptional complexes. Despite similar domain organizations, the two receptors have different coregulator interaction modes. The cryoEM structures now have revealed the fundamental differences between the two receptors and their functional mechanisms.