Open AccesshMSCs possess the potential to differentiate into DP cells in vivo and in vitro
Author(s) -
Wu Minjuan,
Sun Qing,
Guo Xiaocan,
Liu Houqi
Publication year - 2012
Publication title -
cell biology international reports
Language(s) - English
Resource type - Journals
ISSN - 2041-5346
DOI - 10.1042/cbr20120003
Subject(s) - mesenchymal stem cell , microbiology and biotechnology , mesenchyme , versican , cellular differentiation , in vivo , stem cell , chemistry , in vitro , embryonic stem cell , basic fibroblast growth factor , biology , extracellular matrix , growth factor , biochemistry , receptor , proteoglycan , gene
Abstract DP (dermal papilla) is a mesenchyme‐derived structure situated at the base of the HF (hair follicle) that plays an important role in embryonic hair morphogenesis and maintenance of the hair growth cycle. hMSCs (human mesenchymal stem cells) have gained widespread attention in the field of tissue engineering, but not much is known about the differentiation of hMSCs into DP cells. hMSCs involved in HF formation were examined in our previous study. Here, we have explored the differentiation potential of hMSCs into DP cells by co‐culturing hMSCs with DP cells, which proved to be the case. During the differentiation process, the expression of versican, CD133, SCF (stem cell factor), ET‐1 (endothelin‐1) and bFGF (basic fibroblast growth factor) increased. Compared with hMSCs alone, the aggregate number clearly increased when co‐cultured with DP cells. The expression in vivo of HLA‐I (human leucocyte antigen class I) was confined to DP of the newly formed HF. The data suggest that hMSCs possess the potential to differentiate into DP cells in vivo and in vitro .