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Knockdown of OY‐TES‐1 by RNAi causes cell cycle arrest and migration decrease in bone marrow‐derived mesenchymal stem cells
Author(s) -
Cen YanHui,
Guo WenWen,
Luo Bin,
Lin YongDa,
Zhang QingMei,
Zhou SuFang,
Luo GuoRong,
Xiao ShaoWen,
Xie XiaoXun
Publication year - 2012
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20120037
Subject(s) - mesenchymal stem cell , biology , cell cycle , gene knockdown , rna interference , microbiology and biotechnology , bone marrow , cell cycle checkpoint , apoptosis , immunology , rna , gene , genetics
Abstract OY‐TES‐1 is a member of the CTA (cancer‐testis antigen) group expressed in a variety of cancer and restrictedly expressed in adult normal tissues, except for testis. To determine whether MSCs (mesenchymal stem cells) express OY‐TES‐1 and its possible roles on MSCs, OY‐TES‐1 expression in MSCs isolated from human bone marrow was tested with RT (reverse transcription)‐PCR, immunocytochemistry and Western blot. Using RNAi (RNA interference) technology, OY‐TES‐1 expression was knocked down followed by analysing cell viability, cell cycle, apoptosis and migration ability. MSCs expressed OY‐TES‐1 at both mRNA and protein levels. The down‐regulation of OY‐TES‐1 expression in these MSCs caused cell growth inhibition, cell cycle arrest, apoptosis induction and migration ability attenuation. Through these primary results it was suggested that OY‐TES‐1 may influence the biological behaviour of MSCs.