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Vector‐based miR‐15a/16‐1 plasmid inhibits colon cancer growth in vivo
Author(s) -
Dai Lixia,
Wang Wei,
Zhang Shuang,
Jiang Qingyuan,
Wang Ruibo,
Dai Lei,
Cheng Lin,
Yang Yang,
Wei YuQuan,
Deng HongXin
Publication year - 2012
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20110404
Subject(s) - in vivo , angiogenesis , microrna , colorectal cancer , biology , transfection , cancer research , cancer , cancer cell , plasmid , cell growth , microbiology and biotechnology , cell culture , gene , genetics
miR‐15 (microRNA 15) and miR‐16 are frequently deleted or down‐regulated in many cancer cell lines and various tumour tissues, suggesting that miR‐15a/16‐1 plays important roles in tumour progression and might be a method for cancer treatment. We have developed a vector‐based plasmid to explore the anti‐tumour efficacy of miR‐15a/16‐1 in colon cancer in vivo . It is proposed that miR‐15a and miR‐16‐1 target cyclin B1 (CCNB1), which associates with several tumorigenic features such as survival and proliferation. The levels of miR‐15a and miR‐16‐1 in colon cancer cells were inversely correlated with CCNB1 expression, and there was consensus between miR‐15a/16‐1 and CCNB1 mRNA sequences by analysing homology. Vector‐based miR‐15a/16‐1 expression plasmid was constructed and transfected into HCT 116 and SW620 colon cancer cells in vitro . The effects produced on cell viability and angiogenesis were analysed using flow cytometric analysis, colony formation analysis and tube formation analysis. CCNB1 expression down‐regulation was checked by Western blotting. Systemic delivery of miR‐15a/16‐1 plasmids encapsulated in cationic liposome led to a significant inhibition of subcutaneous tumour growth and angiogenesis in tumour tissues, whereas no effects were observed with liposome carrying the non‐specific plasmid. In summary, miR‐15a/16‐1 has been applied in colon cancer treatment in vivo , and resulted in effective colon tumour xenografts growth arrest and angiogenesis decrease. These findings suggest that systemic delivery of vector‐based miR‐15a/16‐1 expression plasmid can be an approach to colon cancer therapy.