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Perforin is recaptured by natural killer cells following target cells stimulation for cytotoxicity
Author(s) -
Wang Lei,
Sun Rulin,
Li Pan,
Han Yang,
Xiong Ping,
Xu Yong,
Fang Min,
Tan Zheng,
Zheng Fang,
Gong Feili
Publication year - 2012
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20110242
Subject(s) - endocytosis , perforin , granzyme b , microbiology and biotechnology , granzyme , cytotoxicity , endosome , biology , cell , biochemistry , in vitro , intracellular
When encountering target cells, NK (natural killer) cells exocytose Pfn (perforin) and granzyme B to kill challengers. We previously reported that granzyme B is recycled and reused by NK cells via clathrin‐dependent endocytosis. However, whether Pfn, a main secretory vesicle content, indispensible to granzyme B killing, undergoes endocytosis remains unknown. We demonstrate that Pfn is recaptured by early endosomes of NK cells via a clathrin‐dependent endocytosis after target cell stimulation. Inhibition of clathrin‐dependent endocytosis significantly attenuated the cytotoxicity of NK cells. The data suggest that the recovery of Pfn contributes to the cytotoxicity of NK cells. The assay of endocytosis of lytic molecule presents a particular focus for exploring the mechanism of abnormal cytotoxicity of NK cells.