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Na + /H + exchanger 1 inhibition contributes to K562 leukaemic cell differentiation
Author(s) -
Jin Weina,
Li Qinghua,
Wang Jian,
Chang Guoqiang,
Lin Yani,
Li Huawen,
Wang Lihong,
Gao Wei,
Pang Tianxiang
Publication year - 2012
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20100919
Subject(s) - chemistry , microbiology and biotechnology , k562 cells , biology , cell , biochemistry
The effect of hypoxia on the differentiation of chronic myeloid leukaemic K562 cells were studied, as was the role of the NHE1 (Na + /H + exchanger 1). Hypoxia induced differentiation of K562 cells as seen by modifications in their morphological features, up‐regulation of C/EBPα (CCAAT/enhancer‐binding protein α), and marked IL‐8 (interleukin‐8) release. Inhibition of NHE1 under hypoxia additionally enhanced the level of C/EBPα and further promoted leukaemic cells differentiation. Pharmacological inhibition of p38 MAPK (mitogen‐activated protein kinase) also significantly suppressed C/EBPα expression under hypoxia conditions after NHE1 inhibition. These results indicate the enhancement of hypoxia‐induced K562 differentiation by NHE1 inhibition, which may be due to up‐regulation of C/EBPα via p38 MAPK signalling pathway, which suggests a possible therapeutic target of NHE1 under hypoxia microenvironment in the treatment of leukaemic diseases.