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Curvature factor and membrane solubilization, with particular reference to membrane rafts
Author(s) -
Mrówczyńska Lucyna,
Salzer Ulrich,
Iglič Aleš,
Hägerstrand Henry
Publication year - 2011
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20100786
Subject(s) - membrane , solubilization , membrane curvature , lipid raft , chemistry , membrane protein , curvature , sphingolipid , elasticity of cell membranes , raft , biophysics , biochemistry , biology , integral membrane protein , lipid bilayer , organic chemistry , geometry , mathematics , copolymer , polymer
The composition of membrane rafts (cholesterol/sphingolipid‐rich domains) cannot be fully deduced from the analysis of a detergent‐resistant membrane fraction after solubilization in Triton X‐100 at 4°C. It is hypothesized that the membrane curvature‐dependent lateral distribution of membrane components affects their solubilization. The stomatocytogenic, Triton X‐100, cannot effectively solubilize membrane components, especially with regard to the outward membrane curvature.