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The hydrolytic activity of esterases in the yeast Saccharomyces cerevisiae is strain dependent
Author(s) -
KwolekMirek Magdalena,
Bednarska Sabina,
Zadra̧gTȩcza Renata,
Bartosz Grzegorz
Publication year - 2011
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20100763
Subject(s) - yeast , saccharomyces cerevisiae , esterase , hydrolysis , biochemistry , strain (injury) , biology , chemistry , saccharomyces , enzyme , anatomy
Ester precursors of fluorogenic or chromogenic probes are often employed in studies of yeast cell biology. This study was aimed at a comparison of the ability of several commonly used laboratory wild‐type Saccharomyces cerevisiae strains to hydrolyse the following model esters: fluorescein diacetate, 2‐naphthyl acetate, PNPA ( p ‐nitrophenyl acetate) and AMQI (7‐acetoxy‐1‐methylquinolinum iodide). In all the strains, the esterase activity was localized mainly to the cytosol. Considerable differences in esterase activity were observed between various wild‐type laboratory yeast strains. The phase of growth also contributed to the variation in esterase activity of the yeast. This diversity implies the need for caution in using intracellularly hydrolysed probes for a comparison of yeast strains with various genetic backgrounds.