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Radiosensitization in human breast carcinoma cells by thymoquinone: role of cell cycle and apoptosis
Author(s) -
VelhoPereira Reelma,
Kumar Amit,
Pandey Badri N.,
Jagtap Aarti G.,
Mishra Kaushala P.
Publication year - 2011
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20100701
Subject(s) - thymoquinone , apoptosis , annexin , cancer research , cell cycle , in vivo , cell growth , in vitro , mcf 7 , chemistry , pharmacology , cancer , cancer cell , medicine , biology , human breast , biochemistry , microbiology and biotechnology , antioxidant
TQ (thymoquinone), the bioactive constituent of black seed ( Nigella sativa ), has been shown to inhibit the growth of various human cancers both in vitro and in vivo . This study reports the radiosensitizing effect of TQ on human breast carcinoma cells (MCF7 and T47D). TQ in combination with single dose of ionizing radiation (2.5 Gy) was found to exert supra‐additive cytotoxic effects on both the carcinomas as measured by cell proliferation and colony‐formation assays. Annexin V binding and FACS analysis revealed the role of enhanced apoptosis and cell cycle modulation in the mechanism of TQ‐mediated radiosensitization, thus supporting TQ as an adjuvant for preclinical testing in cancer chemo‐radiotherapy.