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The human megakaryocytic cell line UT‐7/TPO responds to platelet agonists with intracellular Ca 2+ elevation and P‐selectin expression
Author(s) -
Kawaguchi Tatsuya,
Hashimoto Ryuji,
Nawa Katsuhiko,
Yokota Hiroshi
Publication year - 2011
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20100636
Subject(s) - platelet , platelet activation , thrombin , receptor , thromboxane a2 , chemistry , intracellular , stimulation , thromboxane , signal transduction , medicine , endocrinology , integrin , thromboxane receptor , microbiology and biotechnology , biology , biochemistry
Megakaryocytes have several signal transduction cascades that are similar, but not identical to platelet activation signals. In order to understand platelet signals in detail, it is useful to compare the similarities and/or differences between platelets and megakaryocytes. We evaluated platelet activation signals related to three kinds of Gq protein‐coupled receptors using the megakaryocytic cell line UT‐7/TPO. It was found that UT‐7/TPO responded to thrombin, resulting in a continuous elevation of the [Ca 2+ ]i (intracellular Ca 2+ ) and P‐selectin expression on the surface of the cells. Activation of integrin αIIbβ3 and thromboxane generation was not detected by any of the three stimulations. Taken together, although strong [Ca 2+ ]i elevation by thrombin stimulation caused further P‐selection expression, we could detect [Ca 2+ ]i elevation, which is thought to be the individual signals through the thrombin, thromboxane A 2 or ADP receptor, without considering the secondary signalling caused by αIIbβ3 activation and the arachidonic acid cascade using UT‐7/TPO.