Premium
CCZ1 , MON1 and YPT7 genes are involved in pexophagy, the Cvt pathway and non‐specific macroautophagy in the methylotrophic yeast Pichia pastoris
Author(s) -
Polupanov Andriy S.,
Nazarko Volodymyr Y.,
Sibirny Andriy A.
Publication year - 2011
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20100547
Subject(s) - pichia pastoris , gene , peroxisome , yeast , biology , vacuole , saccharomyces cerevisiae , biochemistry , cytoplasm , recombinant dna
Orthologues of Saccharomyces cerevisiae CCZ1 , MON1 and YPT7 genes in the methylotrophic yeast, Pichia pastoris , have been identified. These genes encode proteins, which act as a complex, being involved in degradation of oleate‐induced peroxisomes, Cvt (cytoplasm to vacuole targeting) pathway and non‐specific macroautophagy in S. cerevisiae . CCZ1 , MON1 and YPT7 gene orthologues are essential for multiple delivery pathways in P. pastoris . Strains with deletion of either of these genes displayed complete deficiency in pexophagy, non‐specific macroautophagy and the biosynthetic Cvt pathway. The data suggest that CCZ1 , MON1 and YPT7 genes are involved in degradation of both small oleate‐induced and large methanol‐induced peroxisomes. The data suggest conservative functions of CCZ1 , MON1 and YPT7 genes among yeast species.