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Mercury chloride increases hepatic alanine aminotransferase and glucose 6‐phosphatase activities in newborn rats in vivo
Author(s) -
MoraesSilva Lucélia,
Bueno Tania Maria,
Franciscato Carina,
Oliveira Cláudia Sirlene,
Peixoto Nilce Coelho,
Pereira Maria Ester
Publication year - 2012
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20100475
Subject(s) - in vivo , alkaline phosphatase , endocrinology , medicine , chemistry , lactate dehydrogenase , phosphatase , mercury (programming language) , kidney , zinc , enzyme , biochemistry , biology , microbiology and biotechnology , organic chemistry , computer science , programming language
This work investigated the in vivo and in vitro effects of HgCl 2 and ZnCl 2 on metabolic enzymes from tissues of young rats to verify whether the physiological and biochemical alterations induced by mercury and prevented by zinc are related to hepatic and renal glucose metabolism. Wistar rats received (subcutaneous) saline or ZnCl 2 (27 mg/kg/day) from 3 to 7 days old and saline or HgCl 2 (5.0 mg/kg/day) from 8 to 12 days old. Mercury exposure increased the hepatic alanine aminotransferase (∼6‐fold) and glucose 6‐phosphatase (75%) activity; zinc pre‐exposure prevented totally and partially these mercury alterations respectively. In vitro , HgCl 2 inhibited the serum (22%, 10 μM) and liver (54%, 100 μM) alanine aminotransferase, serum (53%) and liver (64%) lactate dehydrogenase (10 μM), and liver (53%) and kidney (41%) glucose 6‐phosphatase (100 μM) from 10‐ to 13‐day‐old rats. The results show that mercury induces distinct alterations in these enzymes when tested in vivo or in vitro as well as when different sources were used. The increase of both hepatic alanine aminotransferase and glucose 6‐phosphatase activity suggests that the mercury‐exposed rats have increased gluconeogenic activity in the liver. Zinc prevents the in vivo effects on metabolic changes induced by mercury.