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Effects of extracellular environment factors on the motility in Japanese eel spermatozoa
Author(s) -
Huang Xiaorong,
Zhuang Ping,
Zhang Longzhen,
Qiao Zhenguo,
Jiang Qi,
Liu Jianyi,
Yao Zhifeng,
Feng Guangpeng
Publication year - 2011
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20100474
Subject(s) - milt , motility , sperm motility , sperm , andrology , biology , chemistry , anatomy , microbiology and biotechnology , medicine
We have examined the effects in vitro of the ionic composition, pH and temperature on the motility by the spermatozoa of the Japanese eel ( Anguilla japonica ). Milt was obtained from 10 males that had been artificially matured by repeated injections of hCG. Sperm motility was monitored with a VHS video recorder and a video camera connected to a microscope. The results showed that most of the sperm were highly motile in 250–700 mM NaCl, 250–650 mM KCl and 350–550 mM CaCl 2 solution. The longest duration of sperm motility recorded in 500 mM NaCl, 250 mM KCl and 350 mM CaCl 2 solution. Sperm was not motile when suspended at pH 2, sperm motility was observed at pH 3, there was a relatively higher percentage of motile sperm in solutions at pH 4–12 (above 80%). The motility and duration increased within 18–24°C and decreased at the range of 24–30°C. Appropriate K + ion concentration in the active medium could enhance the percent motility and duration of eel spermatozoa.