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Differentiation of AFS cells derived from the EGFP gene transgenic porcine fetuses
Author(s) -
Zheng YueMao,
Dang YongHui,
Xu YongPing,
Sai WuJiaFu,
An ZhiXing
Publication year - 2011
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20100317
Subject(s) - osteonectin , sox2 , green fluorescent protein , stem cell , biology , microbiology and biotechnology , amniotic fluid , amniotic stem cells , transgene , neural stem cell , amniotic epithelial cells , embryonic stem cell , fetus , adult stem cell , osteocalcin , gene , alkaline phosphatase , genetics , pregnancy , biochemistry , enzyme
We have obtained the EGFP (enhanced green fluorescence protein) gene transgenic porcine fetuses before. The aims of this study were (i) to determine whether stem cells could be isolated from amniotic fluid of the transgenic porcine fetuses, and (ii) to determine if these stem cells could express EGFP and differentiate in vitro . The results demonstrated that stem cells could be isolated from amniotic fluid of the EGFP gene transgenic porcine fetuses and could express EGFP and differentiate in vitro . Undifferentiated AFSs (amniotic fluid‐derived stem cells) expressed POU5F1, THY1 and SOX2, while the following differentiation cells expressed markers for chondrogenic (COL2A1), osteogenic (osteocalcin and osteonectin) and neurogenic cells such as astrocyte (GFAP), oligodendrocyte (GALC) and neuron (NF, ENO2 and MAP).