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Effects of Brn‐4 on the neuronal differentiation of neural stem cells derived from rat midbrain
Author(s) -
Tan XueFeng,
Qin JianBing,
Jin GuoHua,
Tian MeiLing,
Li HaoMing,
Zhu HuiXia,
Zhang XinHua,
Shi JinHong,
Huang Zhen
Publication year - 2010
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20100214
Subject(s) - midbrain , neural stem cell , biology , neuroscience , transcription factor , gene knockdown , cellular differentiation , neural cell , stem cell , microbiology and biotechnology , cell , gene , central nervous system , genetics
NSCs (neural stem cells) provide a powerful research tool for the design and discovery of new approaches to cell replacement therapy during brain repair. However, the usefulness of this tool has been particulaly obstructed by limited neuronal differentiation of NSCs. Brn‐4, a member of the POU domain family of transcription factors, has been previously implicated in the development of neurons by expression analysis. Here, we directly investigated the effects of Brn‐4 on the neuronal differentiation and development of NSCs derived from the E13 rat midbrain. We found that Brn‐4 knockdown in NSCs resulted in a significant decrease of MAP‐2‐positive neurons with immature morphology. Overexpression of Brn‐4 in NSCs markedly increased the production and maturation of newborn neurons. These results suggest that Brn‐4 has a critical role in the neuronal differentiation of mesencephalic NSCs and the maturation of newborn neurons. Brn‐4 may be utilized to manipulate NSCs for gene and cell therapy of several neurological diseases.