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Mesenchymal stem cells isolated from human uterine cervix cancer tissues
Author(s) -
Sun Xiaochun,
Cai Hua,
Qian Hui,
Zhu Wei,
Yan Yongmin,
Xu Huaxi,
Xu Wenrong
Publication year - 2011
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20100132
Subject(s) - mesenchymal stem cell , immunophenotyping , cd90 , cd44 , biology , cd34 , cancer stem cell , pathology , cancer research , cancer cell , stem cell , cancer , flow cytometry , microbiology and biotechnology , cell , medicine , genetics
Abstract In the present study, MSCs (mesenchymal stem cells) were successfully isolated and identified from hUCC (human uterine cervix cancer) tissues. The morphological appearance, immunophenotype, growth curve, cell cycle, cytogenetic features and differentiation potential of these cells were investigated. Results showed that cells isolated from the uterine cervix cancer tissues displayed fibroblast‐like morphology and grew into colonies. Immunophenotyping by flow cytometry revealed that the isolated cells were positive for CD13, CD29, CD44, CD105 and HLA‐I, while negative for CD10, CD14, CD31, CD34, CD38 and HLA‐DR. The cells kept a normal karyotype by chromosome analysis. At the third passage, the percentages of cells in G 0 ‐/G 1 ‐, 2‐/M‐ and S‐phase were 84.94, 8.36 and 6.71%, respectively. Under appropriate induction conditions, these cells can differentiate into osteogenic, adipogenic cells and hepatocytes. Taken together, MSCs were confirmed to exist in hUCC tissues, which may provide a new target for clinical cancer therapy.