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SRY interacts with ribosomal proteins S7 and L13a in nuclear speckles
Author(s) -
Sato Youichi,
Yano Shojiro,
Ewis Ashraf A.,
Nakahori Yutaka
Publication year - 2011
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20090201
Subject(s) - testis determining factor , biology , ribosomal protein , ribosomal rna , microbiology and biotechnology , nuclear protein , gene , y chromosome , genetics , ribosome , rna , transcription factor
The SRY (sex‐determining region on the Y chromosome) is essential for male development; however, the molecular mechanism by which the SRY induces testis development is still unclear. To elucidate the mechanism of testis development, we identified SRY‐interacting proteins using a yeast two‐hybrid system. We found two ribosomal proteins, RPS7 (ribosomal protein S7) and RPL13a (ribosomal protein L13a) that interact with the HMG (high‐mobility group) box domain of SRY. Furthermore, we confirmed the intracellular distributions of RPS7, RPL13a and SRY and found that the three proteins were co‐expressed in COS1 cells. SRY, RPS7 and RPL13a were co‐localized in nuclear speckles. These findings suggest that SRY plays an important role in activities associated with nuclear speckles via an unknown mechanism.