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Characterization of MSCs from human placental decidua basalis in hypoxia and serum deprivation
Author(s) -
Huang YongCan,
Yang ZhiMing,
Jiang NengGang,
Chen XiaoHe,
Li XiuQun,
Tan MeiYun,
Zhou KunPeng,
Tang Li,
Xie HuiQi,
Deng Li
Publication year - 2010
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20090044
Subject(s) - decidua , hypoxia (environmental) , andrology , microbiology and biotechnology , placenta , biology , chemistry , medicine , fetus , pregnancy , genetics , organic chemistry , oxygen
Recently, we reported that human PDB (placental decidua basalis) is an excellent source of MSCs (mesenchymal stem cells), meanwhile, PDB‐MSCs could survive under hypoxia and serum deprivation. Herein, we investigated the proliferation, clonogentic efficiency, phenotypes, metabolic activity and cytokines secretion of PDB‐MSCs in hypoxia and serum deprivation. PDB‐MSCs were cultured in four groups: normoxia (20% O 2 ) and complete medium [10% FBS (foetal bovine serum)+DMEM‐HG (Dulbecco's modified Eagle's medium‐high glucose)], hypoxia and complete medium, normoxia and serum deprivation (0% FBS), and hypoxia and serum deprivation. After 96 h of culture in the above groups, PDB‐MSCs maintain the phenotypes stably. Interestingly, hypoxia notably enhanced the proliferation, colony‐forming potential and lactate/glucose ratio in complete medium, but suppressed the secretion of BMP‐2 (bone morphogenetic protein‐2) and bFGF (basic fibroblast growth factor), while it did not change the quantity of VEGF (vascular endothelial growth factor) and bFGF in serum deprivation. Although PDB‐MSCs grew slowly and seldom formed a colony unit in hypoxia and serum deprivation, they possessed a moderate metabolism. In conclusion, our results indicate that PDB‐MSCs appear to be promising seed cells for ischaemia‐related tissue engineering.