Premium
Pioglitazone suppresses the lipopolysaccharide‐induced production of inflammatory factors in mouse macrophages by inactivating NF‐κB
Author(s) -
Ao Caihui,
Huo Yong,
Qi Litong,
Xiong Zhuowei,
Xue Lin,
Qi Yongfen
Publication year - 2010
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20090005
Subject(s) - pioglitazone , lipopolysaccharide , western blot , tumor necrosis factor alpha , nf κb , chemistry , blot , messenger rna , northern blot , inflammation , microbiology and biotechnology , endocrinology , medicine , biology , biochemistry , gene , diabetes mellitus , type 2 diabetes
TZDs (thiazolidinediones) are prescribed as anti‐Type II diabetes drugs, but little is known regarding whether TZDs regulate the expression of sPLA2 (secretory phospholipase A2) in macrophages. We have investigated the effects of pioglitazone on LPS (lipopolysaccharide)‐induced production of TNF‐α (tumour necrosis factor alpha), sPLA2‐V and ‐X (groups V and X sPLA2) in RAW 264.7 macrophages. TNF‐α, sPLA2‐V and ‐X mRNA and protein expression were determined by RT‐PCR (reverse transcriptase‐PCR) and Western blot analysis, respectively. The activity of NF‐κB (nuclear factor κB) was determined by Western blot and confocal microscopy. LPS induced TNF‐α, sPLA2‐V and sPLA2‐X mRNA and protein expression. Pretreatment with 10 μmol/l pioglitazone significantly suppressed LPS‐induced TNF‐α, sPLA2‐V and sPLA2‐X mRNA and protein expression. LPS induced NF‐κB expression and translocation in the nucleus, but the inductive effects were inhibited by pioglitazone. Our findings indicate that pioglitazone inhibits production of inflammatory factors induced by LPS in murine macrophage cells by inactivating NF‐κB. Pioglitazone appears to play an anti‐inflammatory role in the atherosclerotic process.