Open AccessThe roles of reactive oxygen species and antioxidants in cryopreservation
Author(s) -
Jia Soon Len,
Wen Shuo Darius Koh,
ShiXiong Tan
Publication year - 2019
Publication title -
bioscience reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.938
H-Index - 77
eISSN - 1573-4935
pISSN - 0144-8463
DOI - 10.1042/bsr20191601
Subject(s) - cryopreservation , reactive oxygen species , antioxidant , oxidative stress , oxygen , microbiology and biotechnology , chemistry , biology , biochemistry , embryo , organic chemistry
Cryopreservation has facilitated advancement of biological research by allowing the storage of cells over prolonged periods of time. While cryopreservation at extremely low temperatures would render cells metabolically inactive, cells suffer insults during the freezing and thawing process. Among such insults, the generation of supra-physiological levels of reactive oxygen species (ROS) could impair cellular functions and survival. Antioxidants are potential additives that were reported to partially or completely reverse freeze-thaw stress-associated impairments. This review aims to discuss the potential sources of cryopreservation-induced ROS and the effectiveness of antioxidant administration when used individually or in combination.