Open AccessThe tumor-suppressive function of miR-1296-5p by targeting EGFR and CDK6 in gastric cancer
Author(s) -
Yan Jia,
Lianmei Zhao,
Hanyu Bai,
Cong Zhang,
Suli Dai,
Huilai Lv,
Baoen Shan
Publication year - 2018
Publication title -
bioscience reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.938
H-Index - 77
eISSN - 1573-4935
pISSN - 0144-8463
DOI - 10.1042/bsr20181556
Subject(s) - cyclin dependent kinase 6 , cancer research , cancer , microrna , luciferase , function (biology) , cell growth , cell , cell culture , cancer cell , chemistry , gene , biology , medicine , cell cycle , microbiology and biotechnology , biochemistry , transfection , genetics , cyclin d1
We aimed to confirm the role of miR-1296-5p in gastric cancer and to identify its target genes. The expression of miR-1296-5p was measured in gastric cancer tissues and cell lines. The function of miR-1296-5p was examined by the overexpression and inhibition of its expression in typical gastric cell lines as well as SGC-7901 and MGC-803 cells. The targets of miR-1296-5p were identified by a luciferase activity assay. We found that miR-1296-5p was down-regulated in gastric cancer tissue and cell lines, and low expression levels of miR-1296-5p were associated with advanced clinical stage. Moreover, miR-1296-5p inhibited cell proliferation, migration, and invasion in SGC-7901 and MGC-803 cells. Then, we identified CDK6 and EGFR as novel targets of miR-1296-5p by a luciferase activity assay. Furthermore, the overexpression of miR-1296-5p suppressed the expression of CDK6 and EGFR. Our results indicated a tumor-suppressive role of miR-1296-5p through the translational repression of oncogenic CDK6 and EGFR in gastric cancer.
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