Open AccessSerum microRNAs are non-invasive biomarkers for the presence and progression of subarachnoid haemorrhage
Author(s) -
Niansheng Lai,
Jiaqi Zhang,
Feiyun Qin,
Bin Sheng,
Xinggen Fang,
Zhenbao Li
Publication year - 2017
Publication title -
bioscience reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.938
H-Index - 77
eISSN - 1573-4935
pISSN - 0144-8463
DOI - 10.1042/bsr20160480
Subject(s) - subarachnoid haemorrhage , modified rankin scale , subarachnoid hemorrhage , medicine , pathogenesis , receiver operating characteristic , microrna , microarray , stroke (engine) , gastroenterology , glasgow outcome scale , aneurysm , bioinformatics , pathology , oncology , cardiology , surgery , gene , ischemic stroke , biology , glasgow coma scale , gene expression , mechanical engineering , biochemistry , ischemia , engineering
miRNAs are important regulators of translation and have been associated with the pathogenesis of a number of cardiovascular diseases including stroke and may be possible prognostic biomarkers. The purpose of the present study was to determine the expression levels of miRNAs in the sera of subarachnoid haemorrhage (SAH) patients and to evaluate their relationships with the severity and clinical outcome of SAH. Serum samples on day 3 after the onset of SAH were subjected to microarray analysis with Exqion miRCURY TM LNA array and quantitative PCR analysis. Serum samples from SAH patients ( n =60) and healthy controls ( n =10) were subjected to quantitative PCR analysis. The severities and clinical outcomes of the SAH patients were evaluated with the WFNS grade and the Modified Rankin Scale (mRS). Three miRNAs, miR-502-5p , miR-1297 and miR-4320 were significantly up-regulated in the sera of SAH patients when compared with the healthy controls. The serum miR-502-5p and miR-1297 levels were significantly higher in the patients with severe SAH and a poor outcome than in those with mild SAH and a good outcome ( P <0.05). The areas under the receiver operating characteristic (ROC) curves (AUCs) of miR-502-5p , miR-1297 and miR-4320 to distinguish the SAH patients from the healthy controls were 0.958 ( P <0.001), 0.950 ( P <0.001) and 0.843 ( P <0.001) respectively. Taken together, these results indicate that miR-502-5p and miR-1297 are potentially valuable indicators of the diagnosis, severity and prognosis of SAH, and miR-4320 was a potentially valuable indicator of the diagnosis of SAH.