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RNA interference of metastasis‐associated gene 1 inhibits metastasis of B16F10 melanoma cells in a C57BL/6 mouse model
Author(s) -
Qian Haili,
Yu Jing,
Li Yunfeng,
Wang Haijuan,
Song Chongwen,
Zhang Xueyan,
Liang Xiao,
Fu Ming,
Lin Chen
Publication year - 2007
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1042/bc20060130
Subject(s) - metastasis , melanoma , rna interference , cancer research , biology , carcinogenesis , cancer , gene , rna , genetics
Background information . MTA1 (metastasis‐associated gene 1) has been reported to be overexpressed in cancers with high potential to metastasize. Studies of the molecular mechanisms revealed that MTA1 plays an important role in the process of metastasis of many types of cancer. However, the role of MTA1 in melanoma development is unclear. Results . We have investigated the therapeutic value of MTA1 in the B16F10 melanoma cell line with the C57BL/6 mouse model. Studies in vitro showed that MTA1 promoted the metastatic ability of B16F10 cancer cells. MTA1 down‐regulation by RNA interference greatly reversed the malignant phenotypes of cancer cells. Immunohistochemical staining of MTA1 in human melanoma samples confirmed the up‐regulation of MTA1 in the process of carcinogenesis. Studies in vivo confirmed down‐regulation of MTA1 suppressed the growth and experimental metastasis of B16F10 melanoma cells. Conclusions . MTA1 plays an important role in melanoma development and metastasis. It has a promising potential as a target for in cancer gene therapy or chemotherapy.